Detection of proteolytic activity by covalent tethering of fluorogenic substrates in zymogram gels
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چکیده
منابع مشابه
Detection of proteolytic activity by fluorescent zymogram in-gel assays.
Proteases are involved in the regulation of many biological functions. This study describes a novel method for detecting protease activity by fluorescent zymogram in-gel protease assays, using SDS polyacrylamide gels copolymerized with a peptide-MCA (4-methyl-coumaryl-7-amide) substrate. This method allows simultaneous determination of protease cleavage specificity and molecular weight. Trypsin...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2018
ISSN: 0736-6205,1940-9818
DOI: 10.2144/btn-2018-0005